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mmp-3 polyclonal antibody  (Bioss)


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    Bioss mmp-3 polyclonal antibody
    Mmp 3 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmp-3+polyclonal+antibody/MMP-3+Polyclonal+Antibody/custom%40bs-0413r%4010%2E1016%2Fj%2Ejddst%2E2026%2E108214
    Average 93 stars, based on 27 article reviews
    mmp-3 polyclonal antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Chonggu Granules Improve Cartilage Matrix Metabolism in Knee Osteoarthritis via the miR-148a-3p/Wnt/β-Catenin Pathway
    Article Snippet: ing 0.05% (v/v) Tween-20 (PBST) and cultured in an incubator at 37°C with 0.5% TritonX-100 (B025, Ebiogo, China) and goat serum blocking solution. The goat serum was discarded and the primary antibodies MMP3 (1:200, bs-0413R, Bioss, China), MMP13 (1:200, bs-0575R, Bioss) and Col2aI (1:200, bs-0578R, Bioss) were introduced 60 min before incubation respectively. The sections were then removed, rinsed three times with PBS-T, and infused with the IF s

    Milk:

    Article Title: Chonggu Granules Improve Cartilage Matrix Metabolism in Knee Osteoarthritis via the miR-148a-3p/Wnt/β-Catenin Pathway
    Article Snippet: ing 0.05% (v/v) Tween-20 (PBST) and cultured in an incubator at 37°C with 0.5% TritonX-100 (B025, Ebiogo, China) and goat serum blocking solution. The goat serum was discarded and the primary antibodies MMP3 (1:200, bs-0413R, Bioss, China), MMP13 (1:200, bs-0575R, Bioss) and Col2aI (1:200, bs-0578R, Bioss) were introduced 60 min before incubation respectively. The sections were then removed, rinsed three times with PBS-T, and infused with the IF s

    SDS Page:

    Article Title: Chonggu Granules Improve Cartilage Matrix Metabolism in Knee Osteoarthritis via the miR-148a-3p/Wnt/β-Catenin Pathway
    Article Snippet: ing 0.05% (v/v) Tween-20 (PBST) and cultured in an incubator at 37°C with 0.5% TritonX-100 (B025, Ebiogo, China) and goat serum blocking solution. The goat serum was discarded and the primary antibodies MMP3 (1:200, bs-0413R, Bioss, China), MMP13 (1:200, bs-0575R, Bioss) and Col2aI (1:200, bs-0578R, Bioss) were introduced 60 min before incubation respectively. The sections were then removed, rinsed three times with PBS-T, and infused with the IF s



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    Real-time PCR data indicated that supplementation with α2MRS reduced cartilage matrix catabolism and enhanced anabolism in-vitro (A–D) and in-vivo (E–J) . mRNA levels of <t>MMP-3</t> ( p < 0.001), MMP-13 ( p < 0.001), Col-10 a1 ( p = 0.001), and Runx-2 ( p < 0.001)were lower in IL-1β+α2MRS and IACL-R+α2MRS groups than in IL-1β and IACL-R groups respectively. In contrast, mRNA levels of Col-2 ( p < 0.001) and aggrecan ( p < 0.001) followed the opposite pattern. Both were increased in IL-1β+α2MRS and IACL-R+α2MRS groups as compared to levels in IL-1β and IACL-R groups. * = p < 0.05, control group versus IL-1β group or sham group versus IACL-R group; # = p < 0.05, control group versus IL-1β+α2MRS group or sham group versus IACL-R+α2MRS group; and = p < 0.05, IL-1β group versus IL-1β+α2MRS group or IACL-R group versus IACL-R+α2MRS group. The bars show the mean ± SD ( n = 6).
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    Image Search Results


    Total flavonoids inhibit the p38 and JNK-mediated MAPK pathways in photoaged human dermal fibroblasts. (a)–(e) Western blot to check the effect of total flavonoids on MMP-1, MMP-3, p-JNK, and p-p38 in photoaged human dermal fibroblasts. ∗ P < 0.05, ∗∗ P < 0.01.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Protective Effects and Molecular Mechanism of Total Flavonoids from Lycium Barbarum Leaves on Photoaged Human Dermal Fibroblasts

    doi: 10.1155/2022/4156330

    Figure Lengend Snippet: Total flavonoids inhibit the p38 and JNK-mediated MAPK pathways in photoaged human dermal fibroblasts. (a)–(e) Western blot to check the effect of total flavonoids on MMP-1, MMP-3, p-JNK, and p-p38 in photoaged human dermal fibroblasts. ∗ P < 0.05, ∗∗ P < 0.01.

    Article Snippet: The membrane was blocked with 5% skimmed milk blocking solution for 2 h. After blocking, indicated primary antibodies (anti-MMP-1 (bioss, bs-0424R-50), anti-MMP-3 (bioss, bs-0413R-50), phospho-JNK1/2/3 (Affinity Biosciences, AF3318-50), phospho-p38 MAPK (Affinity Biosciences, KAF4001-50), and GAPDH (ab8245, Abcam)) were added for incubation at 4°C overnight.

    Techniques: Western Blot

    Real-time PCR data indicated that supplementation with α2MRS reduced cartilage matrix catabolism and enhanced anabolism in-vitro (A–D) and in-vivo (E–J) . mRNA levels of MMP-3 ( p < 0.001), MMP-13 ( p < 0.001), Col-10 a1 ( p = 0.001), and Runx-2 ( p < 0.001)were lower in IL-1β+α2MRS and IACL-R+α2MRS groups than in IL-1β and IACL-R groups respectively. In contrast, mRNA levels of Col-2 ( p < 0.001) and aggrecan ( p < 0.001) followed the opposite pattern. Both were increased in IL-1β+α2MRS and IACL-R+α2MRS groups as compared to levels in IL-1β and IACL-R groups. * = p < 0.05, control group versus IL-1β group or sham group versus IACL-R group; # = p < 0.05, control group versus IL-1β+α2MRS group or sham group versus IACL-R+α2MRS group; and = p < 0.05, IL-1β group versus IL-1β+α2MRS group or IACL-R group versus IACL-R+α2MRS group. The bars show the mean ± SD ( n = 6).

    Journal: Frontiers in Pharmacology

    Article Title: α2-macroglobulin-rich serum as a master inhibitor of inflammatory factors attenuates cartilage degeneration in a mini pig model of osteoarthritis induced by “idealized” anterior cruciate ligament reconstruction

    doi: 10.3389/fphar.2022.849102

    Figure Lengend Snippet: Real-time PCR data indicated that supplementation with α2MRS reduced cartilage matrix catabolism and enhanced anabolism in-vitro (A–D) and in-vivo (E–J) . mRNA levels of MMP-3 ( p < 0.001), MMP-13 ( p < 0.001), Col-10 a1 ( p = 0.001), and Runx-2 ( p < 0.001)were lower in IL-1β+α2MRS and IACL-R+α2MRS groups than in IL-1β and IACL-R groups respectively. In contrast, mRNA levels of Col-2 ( p < 0.001) and aggrecan ( p < 0.001) followed the opposite pattern. Both were increased in IL-1β+α2MRS and IACL-R+α2MRS groups as compared to levels in IL-1β and IACL-R groups. * = p < 0.05, control group versus IL-1β group or sham group versus IACL-R group; # = p < 0.05, control group versus IL-1β+α2MRS group or sham group versus IACL-R+α2MRS group; and = p < 0.05, IL-1β group versus IL-1β+α2MRS group or IACL-R group versus IACL-R+α2MRS group. The bars show the mean ± SD ( n = 6).

    Article Snippet: The following antibodies were used in this study: collagen-2 (Col-2, ab34712, Abcam), matrix metalloproteinase -3 (MMP-3, bs-0413R, Bioss), MMP-9 (bs-4593R, Bioss), MMP-13 (K009743P, Solarbio), Col-10 (bs-0554R, Bioss), and Runt-related transcription factor 2 (Runx-2, ab76956, Abcam).

    Techniques: Real-time Polymerase Chain Reaction, In Vitro, In Vivo, Control

    (A) : Articular cartilage immunostaining showed that MMP-3, MMP-9, MMP-13, Runx-2, and Col -10 staining significantly increased in the IACL-R mini pig group but were lower in the IACL-R+α2MRS group. In contrast, Col-2 expression in articular cartilage was higher in the IACL-R+α2MRS group than in the IACL-R group. (B) Quantitative immunohistochemical analysis of articular cartilage. (C) synovium immunostaining showed that MMP-3, MMP-9 and MMP-13 staining significantly increased in the IACL-R mini pig group but were lower in the IACL-R+α2MRS group. (D) Quantitative immunohistochemical analysis of synovium. * = p < 0.05, sham group versus IACL-R group; # = p < 0.05, sham group versus IACL-R+α2MRS group; and = p < 0.05, IACL-R group versus IACL-R+α2MRS group. The bars show the mean ± SD ( n = 6).

    Journal: Frontiers in Pharmacology

    Article Title: α2-macroglobulin-rich serum as a master inhibitor of inflammatory factors attenuates cartilage degeneration in a mini pig model of osteoarthritis induced by “idealized” anterior cruciate ligament reconstruction

    doi: 10.3389/fphar.2022.849102

    Figure Lengend Snippet: (A) : Articular cartilage immunostaining showed that MMP-3, MMP-9, MMP-13, Runx-2, and Col -10 staining significantly increased in the IACL-R mini pig group but were lower in the IACL-R+α2MRS group. In contrast, Col-2 expression in articular cartilage was higher in the IACL-R+α2MRS group than in the IACL-R group. (B) Quantitative immunohistochemical analysis of articular cartilage. (C) synovium immunostaining showed that MMP-3, MMP-9 and MMP-13 staining significantly increased in the IACL-R mini pig group but were lower in the IACL-R+α2MRS group. (D) Quantitative immunohistochemical analysis of synovium. * = p < 0.05, sham group versus IACL-R group; # = p < 0.05, sham group versus IACL-R+α2MRS group; and = p < 0.05, IACL-R group versus IACL-R+α2MRS group. The bars show the mean ± SD ( n = 6).

    Article Snippet: The following antibodies were used in this study: collagen-2 (Col-2, ab34712, Abcam), matrix metalloproteinase -3 (MMP-3, bs-0413R, Bioss), MMP-9 (bs-4593R, Bioss), MMP-13 (K009743P, Solarbio), Col-10 (bs-0554R, Bioss), and Runt-related transcription factor 2 (Runx-2, ab76956, Abcam).

    Techniques: Immunostaining, Staining, Expressing, Immunohistochemical staining

    Antibodies.

    Journal: Frontiers in Immunology

    Article Title: IL-22 regulates endometrial regeneration by enhancing tight junctions and orchestrating extracellular matrix

    doi: 10.3389/fimmu.2022.955576

    Figure Lengend Snippet: Antibodies.

    Article Snippet: 2 , MMP-3, rabbit polyclonal , Bioss, MA, USA , bs-0413R , 1:1,000.

    Techniques: Concentration Assay